Подумалось что стоит завести отдельную тему, т.к. информация размазана по разным местам...
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http://www.microscopy-uk.org.uk/mag/ind ... tips5.html
Цитата:
I have done some more testing and some thinking about why the image quality was so poor when the condenser was oiled and the aperture was set to fill about 80% of the rear element of the 90X objective. I believe that the poor image quality is the result of oiling the condenser. The image quality is good when the condenser is dry. Köhler illumination can then be achieved with the 90X objective, but only with a dry condenser.
The best illumination is with a 245 mm distance from the collector lens to the condenser with about 0.70 mm air gap under the slide to the top of the condenser. If you think about what happens, if there is a significant gap you will see how the spherical aberration of the illumination system would be drastically increased when this gap is filled with oil. The condenser is claimed to be aplanatic. I maintain that it is aplanatic at high NA, but only when used dry.
The LOMO information indicates that the condenser is expected to normally be used dry. The claimed 1.40 NA can only be achieved when the air gap below the slide is filled with oil, but then the image quality is ruined by the spherical aberration in the illumination caused by oiling the condenser. I believe that this illumination with an oiled condenser will be very useful for darkfield using the water immersion 30X objective without a cover glass.
The best illumination is with a 245 mm distance from the collector lens to the condenser with about 0.70 mm air gap under the slide to the top of the condenser. If you think about what happens, if there is a significant gap you will see how the spherical aberration of the illumination system would be drastically increased when this gap is filled with oil. The condenser is claimed to be aplanatic. I maintain that it is aplanatic at high NA, but only when used dry.
The LOMO information indicates that the condenser is expected to normally be used dry. The claimed 1.40 NA can only be achieved when the air gap below the slide is filled with oil, but then the image quality is ruined by the spherical aberration in the illumination caused by oiling the condenser. I believe that this illumination with an oiled condenser will be very useful for darkfield using the water immersion 30X objective without a cover glass.
Получается, лучше его не мазать?
